4FYP
Crystal Structure of Plant Vegetative Storage Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Collection date | 2012-04-13 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.328, 49.182, 84.905 |
| Unit cell angles | 90.00, 116.09, 90.00 |
Refinement procedure
| Resolution | 30.460 - 1.800 |
| R-factor | 0.1833 |
| Rwork | 0.181 |
| R-free | 0.22440 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.355 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.027 | 0.421 | |
| Number of reflections | 41290 | ||
| Completeness [%] | 97.2 | 96.6 | 96.2 |
| Redundancy | 4.2 | 4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | sodium citrate tribasic dihydrate, 2-propanol, polyethylene glycol 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
