4FXZ
Crystal structure of LeuT-F253A bound to L-leucine from lipid bicelles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.970 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.140, 89.888, 81.262 |
| Unit cell angles | 90.00, 102.95, 90.00 |
Refinement procedure
| Resolution | 36.298 - 2.601 |
| R-factor | 0.2136 |
| Rwork | 0.213 |
| R-free | 0.22030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.542 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.088 | 0.535 |
| Number of reflections | 25839 | |
| <I/σ(I)> | 4.8 | 2 |
| Completeness [%] | 97.9 | 98.6 |
| Redundancy | 5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | 100 mM NaAC, 29%MPD, 10%PEG400, 50 mM MgCl2, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
