4FXV

Crystal structure of an ELAV-like protein 1 (ELAVL1) from Homo sapiens at 1.90 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-15
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 61 2 2
Unit cell lengths	73.436, 73.436, 272.957
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.823 - 1.900
R-factor	0.2032
Rwork	0.202
R-free	0.23490
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.781
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SHELX
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.823	28.823	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge		0.058	0.482
Rmeas	0.140	0.062	0.525
Rpim	0.044	0.020	0.199
Total number of observations	352640	4004	15252
Number of reflections	34829
<I/σ(I)>	11.2	20.4	2.6
Completeness [%]	97.9	95.8	87.4
Redundancy	10.1	8.1	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.3	277	0.200M NH4Cl, 20.00% PEG-3350, No Buffer pH 6.3, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K