4FXB
Crystal structure of CYP105N1 from Streptomyces coelicolor: a cytochrome P450 oxidase in the coelibactin siderophore biosynthetic pathway
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-19 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 133.086, 133.086, 227.111 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.930 - 2.900 |
| R-factor | 0.28238 |
| Rwork | 0.281 |
| R-free | 0.30235 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e5j |
| RMSD bond length | 0.026 |
| RMSD bond angle | 3.499 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 98528 | |
| Completeness [%] | 88.8 | 88.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 287 | 0.1M imidazole, 0.2M sodium chloride, 30%(w/v) PEG8000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 287.0K |
