4FWV
Crystal structure of the N-terminal domain of the Lon-like protease MtaLonC
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9639, 0.9789 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 147.683, 147.683, 163.851 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.2025 |
| Rwork | 0.201 |
| R-free | 0.22906 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.518 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (AutoSol) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.079 | 0.925 |
| Number of reflections | 26977 | |
| <I/σ(I)> | 17.8 | 1.9 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 5.5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 295 | 400mM lithium sulphate, 10mM nickel chloride, 100mM Tris-HCl, pH 8.4, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
