4FWH
Crystal structure of the Lon-like protease MtaLonC in complex with MG262
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 6 |
| Unit cell lengths | 115.995, 115.995, 136.124 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.190 |
| R-factor | 0.17619 |
| Rwork | 0.174 |
| R-free | 0.22675 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fw9 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.366 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.091 | 0.852 |
| Number of reflections | 59267 | |
| <I/σ(I)> | 40.1 | 4.4 |
| Completeness [%] | 97.9 | 94.6 |
| Redundancy | 10.2 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | 10% isopropanol, 200mM di-potassium phosphate, 100mM sodium citrate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
