4FVG
SPFH domain of mouse stomatin (Crystal form 3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-15 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.918410 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 84.489, 84.489, 68.937 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 73.170 - 1.800 |
| R-factor | 0.19592 |
| Rwork | 0.194 |
| R-free | 0.24512 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fvf |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.986 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.170 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.041 | 0.871 |
| Number of reflections | 16492 | |
| <I/σ(I)> | 33.25 | 2.97 |
| Completeness [%] | 99.7 | 98.7 |
| Redundancy | 11.6 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 5% ethanol, 0.02 cadmium sulfate, 0.1 HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
