4FV7
Crystal Structure of the ERK2 complexed with E94
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.630, 70.190, 61.120 |
| Unit cell angles | 90.00, 111.20, 90.00 |
Refinement procedure
| Resolution | 27.100 - 1.900 |
| R-factor | 0.1843 |
| Rwork | 0.183 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.940 |
| Data scaling software | SCALEPACK |
| Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 55.000 | 55.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 4.120 | 1.800 |
| Rmerge | 0.046 | 0.026 | 0.426 |
| Number of reflections | 33424 | ||
| <I/σ(I)> | 18.6 | ||
| Completeness [%] | 93.2 | 97.9 | 78.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 100 mM MES buffer, pH 6.5, 26-28% PEG-MME 2000, 200 mM ammonium sulfate and 20 mM 2-mercaptoethanol, vapor diffusion, temperature 298K |
