4FV1
Crystal Structure of the ERK2 complexed with EK4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.750, 70.070, 60.330 |
Unit cell angles | 90.00, 109.95, 90.00 |
Refinement procedure
Resolution | 38.350 - 1.990 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.22450 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.910 |
Data scaling software | SCALEPACK |
Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 55.000 | 55.000 | 2.060 |
High resolution limit [Å] | 1.990 | 4.580 | 1.990 |
Rmerge | 0.052 | 0.040 | 0.185 |
Number of reflections | 25736 | ||
<I/σ(I)> | 20.2 | ||
Completeness [%] | 98.2 | 95.5 | 87.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | 100 mM MES buffer, pH 6.5, 26-28% PEG-MME 2000, 200 mM ammonium sulfate and 20 mM 2-mercaptoethanol, vapor diffusion, temperature 298K |