4FUO
Structural basis for Zn2+-dependent intercellular adhesion in staphylococcal biofilms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.105, 34.619, 49.369 |
| Unit cell angles | 90.00, 103.11, 90.00 |
Refinement procedure
| Resolution | 25.980 - 1.970 |
| R-factor | 0.2095 |
| Rwork | 0.207 |
| R-free | 0.25740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 1.960 | 4.220 | 1.960 |
| Rmerge | 0.083 | 0.063 | 0.537 |
| Number of reflections | 13864 | ||
| <I/σ(I)> | 14.7 | ||
| Completeness [%] | 95.6 | 94.6 | 75.9 |
| Redundancy | 5.2 | 5.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 7.9 | 293 | 0.06 M Postassium Thiocyanate, 0.1 M Tris pH 7.9, 28% PEG MME 2000, 2% PEG 3350, batch, temperature 293K |
