4FUN
Structural basis for Zn2+-dependent intercellular adhesion in staphylococcal biofilms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2822 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.034, 33.564, 83.148 |
| Unit cell angles | 90.00, 122.47, 90.00 |
Refinement procedure
| Resolution | 46.010 - 2.310 |
| R-factor | 0.2055 |
| Rwork | 0.203 |
| R-free | 0.25780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.390 |
| High resolution limit [Å] | 2.310 | 4.980 | 2.310 |
| Rmerge | 0.102 | 0.078 | 0.275 |
| Number of reflections | 9395 | ||
| <I/σ(I)> | 20 | ||
| Completeness [%] | 97.6 | 97.3 | 96 |
| Redundancy | 7.3 | 7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 7.9 | 293 | 0.06 M Postassium Thiocyanate, 0.1 M Tris pH 7.9, 28% PEG MME 2000, 2% PEG 3350, batch, temperature 293K |
