4FUM
Structural basis for Zn2+-dependent intercellular adhesion in staphylococcal biofilms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH, INVERSE BEAM |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.721, 33.637, 81.325 |
| Unit cell angles | 90.00, 121.06, 90.00 |
Refinement procedure
| Resolution | 44.820 - 2.400 |
| R-factor | 0.2006 |
| Rwork | 0.198 |
| R-free | 0.25530 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX (Autosol) |
| Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.111 |
| Number of reflections | 8282 |
| Completeness [%] | 99.3 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 7.9 | 293 | 0.12 M Postassium Thiocyanate, 0.1 M Tris pH 7.9, 28% PEG MME 2000, 3% Ethanol, batch, temperature 293K |
