4FU8

Crystal Structure of the Urokinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	160
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	55.299, 52.782, 79.787
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.950 - 2.200
R-factor	0.1948
Rwork	0.193
R-free	0.23930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.910
Refinement software	BUSTER-TNT (BUSTER 2.11.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.950
High resolution limit [Å]	2.197
Number of reflections	11520
Completeness [%]	92.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.15 M Li2SO4, 20% polyethylene glycol MW 4000 in succinate buffer, pH 4.8-6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K