4FU3

CID of human RPRD1B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Detector technology	CCD
Collection date	2011-10-19
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9179
Spacegroup name	C 2 2 21
Unit cell lengths	57.752, 79.784, 108.666
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.770 - 1.900
R-factor	0.2116
Rwork	0.209
R-free	0.25890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	unpublished model of same protein different crystal form
RMSD bond length	0.010
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	SCALA (CCP4_3.3.20)
Phasing software	MOLREP
Refinement software	BUSTER-TNT (BUSTER 2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.780	46.780	2.000
High resolution limit [Å]	1.900	6.010	1.900
Rmerge	0.070	0.030	0.980
Total number of observations		4163	20438
Number of reflections	20170
<I/σ(I)>	19.6638
Completeness [%]	100.0	98.82	99.96
Redundancy	7.19	5.95	7.11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	291	30% PEG-4000, 0.2M magnesium chloride, 0.1M TRIS hydrochloride, pH 8.5, vapor diffusion, temperature 291K
1	VAPOR DIFFUSION	8.5	291	30% PEG-4000, 0.2M magnesium chloride, 0.1M TRIS hydrochloride, pH 8.5, vapor diffusion, temperature 291K