4FTV
The complex between the high affinity version of A6 TCR (A6c134) and human Class I MHC HLA-A2 with the bound TAX nonameric peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-12 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.98494 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 224.230, 48.954, 94.398 |
| Unit cell angles | 90.00, 89.62, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.740 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.26040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.378 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.095 | 0.557 |
| Number of reflections | 26730 | |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | PEG3350 24%, MES 0.025M, NaCl 0.1M, pH 6.5, vapor diffusion, sitting drop, temperature 277K |
