4FTF
Structure of the Type II secretion system pilotin AspS from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 34.160, 63.860, 47.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.540 - 1.480 |
| R-factor | 0.1561 |
| Rwork | 0.155 |
| R-free | 0.17650 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.529 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.540 | 1.520 | |
| High resolution limit [Å] | 1.480 | 6.620 | 1.480 |
| Rmerge | 0.084 | 0.030 | 0.958 |
| Number of reflections | 17689 | 371 | 2074 |
| <I/σ(I)> | 16.17 | 48.96 | 1.43 |
| Completeness [%] | 98.5 | 100 | 84.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.2M ZINC ACETATE, 20% PEG 3350, pH 7.5, vapor diffusion, hanging drop, temperature 295K |
