4FSX
crystal structure of Se-substituted Zea mays ZMET2 in complex with SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 |
Unit cell lengths | 64.857, 88.952, 113.493 |
Unit cell angles | 93.47, 95.53, 110.41 |
Refinement procedure
Resolution | 40.250 - 3.200 |
R-factor | 0.241 |
Rwork | 0.240 |
R-free | 0.26300 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.819 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.175 | 0.595 |
Number of reflections | 38702 | |
<I/σ(I)> | 7.2 | 1.6 |
Completeness [%] | 99.2 | 98.8 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.2 M calcium acetate, 0.1 M imidazole pH 8.0, and 10% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |