4FSV

Crystal structure of a heat shock 70kDa protein 2 (HSPA2) from Homo sapiens at 1.80 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-11
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97946,0.97931
Spacegroup name	P 21 21 21
Unit cell lengths	49.116, 82.285, 95.802
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.773 - 1.800
R-factor	0.1687
Rwork	0.167
R-free	0.19790
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.421
Data reduction software	XDS
Data scaling software	XSCALE (December 29, 2011)
Phasing software	SHELX
Refinement software	REFMAC (5.6.0116)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.773		1.860
High resolution limit [Å]	1.800	3.870	1.800
Rmerge	0.056	0.035	0.446
Number of reflections	36451	6515	6433
<I/σ(I)>	8.93	21.4	1.7
Completeness [%]	97.8	92.5	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	20.00% 2-methyl-2,4-pentanediol, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K