4FSP
Crystal Structure of Pseudomonas aeruginosa OccK11 (OpdR)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-19 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.897 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 95.892, 147.810, 84.513 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.948 - 2.323 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.24060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t20 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.232 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Number of reflections | 26201 | |
| <I/σ(I)> | 33.1 | 4.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 10% PEG 4000, 0.2M potassium fluoride, 0.05M sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
