4FSO
Crystal Structure of Pseudomonas aeruginosa OccK10 (OpdN)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.872, 87.081, 98.177 |
| Unit cell angles | 90.00, 98.21, 90.00 |
Refinement procedure
| Resolution | 14.800 - 2.750 |
| R-factor | 0.22 |
| Rwork | 0.216 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t20 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 25528 | |
| <I/σ(I)> | 13 | 1.8 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 293 | 10% PEG 4000, 0.2M calcium chloride, 0.05M sodium citrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
