4FS7

Crystal structure of a leucine-rich repeat protein (BACOVA_04585) from Bacteroides ovatus ATCC 8483 at 1.19 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-11
Detector	MAR MAR325
Wavelength(s)	0.91837,0.97949,0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	36.199, 84.416, 121.937
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.278 - 1.190
R-factor	0.132
Rwork	0.131
R-free	0.15700
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.627
Data reduction software	XDS
Data scaling software	XSCALE (December 29, 2011)
Phasing software	SHELX
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.278		1.230
High resolution limit [Å]	1.190	2.560	1.190
Rmerge	0.048	0.028	0.513
Rmeas	0.061	0.035	0.677
Total number of observations	430225
Number of reflections	119592	20933	21084
<I/σ(I)>	9.11	25.8	1.6
Completeness [%]	97.8	89.8	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			293	0.200M sodium fluoride 20.00% polyethylene glycol 3350, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K