4FRZ

Arabidopsis KCBP motor domain dimerized via regulatory domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	200
Detector technology	CCD
Collection date	2008-11-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1
Spacegroup name	P 1 21 1
Unit cell lengths	45.713, 75.068, 120.598
Unit cell angles	90.00, 91.45, 90.00

Refinement procedure

Resolution	25.000 - 2.400
R-factor	0.2629
Rwork	0.225
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sdm
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	29651
<I/σ(I)>		2.41
Completeness [%]	91.9	81.9
Redundancy		2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	10% PEG 3000, 100 mM imidazole (pH 8.0), 200 mM Li2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 277K