4FRZ
Arabidopsis KCBP motor domain dimerized via regulatory domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2008-11-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.713, 75.068, 120.598 |
| Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.400 |
| R-factor | 0.2629 |
| Rwork | 0.225 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sdm |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 29651 | |
| <I/σ(I)> | 2.41 | |
| Completeness [%] | 91.9 | 81.9 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 10% PEG 3000, 100 mM imidazole (pH 8.0), 200 mM Li2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
