4FRY
The structure of a putative signal-transduction protein with CBS domains from Burkholderia ambifaria MC40-6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-18 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.350, 56.630, 76.580 |
Unit cell angles | 90.00, 98.41, 90.00 |
Refinement procedure
Resolution | ? - 2.100 |
R-factor | 0.2252 |
Rwork | 0.223 |
R-free | 0.26690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rc3 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.563 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 31.485 | 2.150 | |
High resolution limit [Å] | 2.100 | 9.390 | 2.100 |
Rmerge | 0.073 | 0.036 | 0.381 |
Number of reflections | 16262 | 187 | 1072 |
<I/σ(I)> | 9.8 | 21.31 | 2.68 |
Completeness [%] | 97.9 | 92.6 | 90.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 40.6 mg/ml BuamA.00062.b.A1.PS01259, 20% PEG 3350, 200mM potassium nitrate, cryo protection 20% PEG 300, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |