4FRN
Crystal structure of the cobalamin riboswitch regulatory element
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-05 |
| Detector | ADSC 315r |
| Wavelength(s) | 1.60 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.605, 81.833, 147.722 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.830 - 3.430 |
| R-factor | 0.252 |
| Rwork | 0.251 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4frg |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 55.605 | 55.600 | 3.620 |
| High resolution limit [Å] | 3.430 | 10.850 | 3.430 |
| Rmerge | 0.020 | 0.298 | |
| Total number of observations | 1012 | 4656 | |
| Number of reflections | 9466 | ||
| <I/σ(I)> | 8.512 | 26.3 | 2.6 |
| Completeness [%] | 99.5 | 96.4 | 99.5 |
| Redundancy | 3.4 | 3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7 | 298 | 10% v/v MPD, 40 mM sodium cacodylate pH 7.0, 12 mM spermine tetrahydrochloride, 80 mM potassium chloride, 20 mM barium chloride, hanging drop, temperature 298K |
