4FRF
Structural Studies and Protein Engineering of Inositol Phosphate Multikinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 130.790, 130.790, 129.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.000 - 2.900 |
| R-factor | 0.23935 |
| Rwork | 0.239 |
| R-free | 0.25099 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.922 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.890 |
| Rmerge | 0.085 | 0.540 |
| Number of reflections | 28740 | |
| <I/σ(I)> | 2.25 | |
| Completeness [%] | 100.0 | 93.4 |
| Redundancy | 10.95 | 10.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 2.0M Ammonium sulfate 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
