4FR4
Crystal structure of human serine/threonine-protein kinase 32A (YANK1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.91730 |
| Spacegroup name | P 32 |
| Unit cell lengths | 154.110, 154.110, 112.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.440 - 2.290 |
| R-factor | 0.20636 |
| Rwork | 0.204 |
| R-free | 0.24449 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a8x 3L9N and 3G51 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.488 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.680 | 2.410 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.089 | 0.638 |
| Number of reflections | 128336 | |
| <I/σ(I)> | 9.3 | 2 |
| Completeness [%] | 95.7 | 97.8 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 277 | 20% PEG 3350, 0.2M sodium acetate, 10% ethylene glycol, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
