Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 67.183, 128.789, 96.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.750 |
| R-factor | 0.20945 |
| Rwork | 0.206 |
| R-free | 0.28338 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fs8 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.623 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.870 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.098 | 0.485 |
| Number of reflections | 11298 | |
| <I/σ(I)> | 3.7 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 5.9 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.2 | 298 | 20% PEG 8000, 0.2 M NaCl, 0.1 M Citrate Phosphate, 10 mM TCEP, pH 4.2, EVAPORATION, temperature 298.0K |
