4FQU
Glutathionyl-Hydroquinone Reductase PcpF of Sphingobium chlorophenolicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 242.845, 242.845, 242.845 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.571 - 3.000 |
| R-factor | 0.203 |
| Rwork | 0.202 |
| R-free | 0.25590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.860 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.600 | 3.049 |
| High resolution limit [Å] | 2.800 | 3.000 |
| Number of reflections | 162729 | |
| Completeness [%] | 91.3 | 72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 1.6M ammonium sulfate, 0.1M MES monohydrate, 10% v/v 1,4-dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
