4FQN

Crystal structure of the CCM2 C-terminal Harmonin Homology Domain (HHD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-30
Detector	PSI PILATUS 6M
Wavelength(s)	0.9778
Spacegroup name	P 21 21 21
Unit cell lengths	62.166, 64.196, 89.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 1.900
R-factor	0.1848
Rwork	0.183
R-free	0.21900
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.012
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (autosol)
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Number of reflections	29059
<I/σ(I)>	13.7	30.3	1.6
Completeness [%]	100.0	100	100
Redundancy	11.8	11.8	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	2.072M ammonium sulfate, 0.2M potassium formate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K