4FQM

Structure of B/Brisbane/60/2008 Influenza Hemagglutinin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Detector technology	CCD
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97975
Spacegroup name	P 21 21 2
Unit cell lengths	138.690, 242.540, 135.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.066 - 3.450
R-factor	0.2577
Rwork	0.257
R-free	0.26750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rft
RMSD bond length	0.011
RMSD bond angle	1.610
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	135.066	3.550
High resolution limit [Å]	3.450	3.450
Number of reflections	60737
<I/σ(I)>	11.6
Completeness [%]	99.7	91.8
Redundancy	7.4	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	295	20% PEG3350, 160 mM sodium fluoride, 20 mM Tris, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K