4FQD
Crystal structure of the enolpyruvyl transferase NikO from Streptomyces tendae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 96.210, 120.050, 153.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.248 - 2.500 |
| R-factor | 0.1972 |
| Rwork | 0.195 |
| R-free | 0.23280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yvw |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.631 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.070 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.099 | 0.270 |
| Number of reflections | 28377 | |
| <I/σ(I)> | 13 | 6.6 |
| Completeness [%] | 91.3 | 91.1 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 289 | 0.2 M Li Sulfate, 0.1 M Bis-Tris pH 6.5, 25% w/v PEG 3350, Microbatch, temperature 289K |
