4FQB
crystal structure of toxic effector Tse1 in complex with immune protein Tsi1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-01 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0090 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 97.710, 97.710, 293.301 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.690 |
| R-factor | 0.20337 |
| Rwork | 0.201 |
| R-free | 0.24546 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fqa |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.810 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.840 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 1.100 | |
| Number of reflections | 43188 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 293 | 100mM Sodium Citrate, 20% V/V 2-propanol, 20% W/V PEG4000,1mM DTT, 3% V/V Ethylene Glycol, pH 5.6, VAPOR DIFFUSION, temperature 293K |
