4FQ2
Crystal Structure of 10-1074 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.383, 40.261, 84.460 |
| Unit cell angles | 90.00, 95.39, 90.00 |
Refinement procedure
| Resolution | 36.313 - 1.900 |
| R-factor | 0.1885 |
| Rwork | 0.187 |
| R-free | 0.22290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.234 |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 36.313 | 36.313 | 1.800 |
| High resolution limit [Å] | 1.704 | 5.390 | 1.700 |
| Rmerge | 0.027 | 0.849 | |
| Number of reflections | 42465 | ||
| <I/σ(I)> | 8.7 | 21.3 | 0.9 |
| Completeness [%] | 94.0 | 92.7 | 80.6 |
| Redundancy | 2.7 | 2.8 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 298 | PEG 3350, Bis-Tris HCl, NaCl, pH 5.5, vapor diffusion, temperature 298K |
