4FPQ

Structure of a fungal protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 43
Unit cell lengths	60.830, 60.830, 155.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.315 - 2.300
R-factor	0.2468
Rwork	0.244
R-free	0.30820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.083
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.430
High resolution limit [Å]	2.300	2.300
Rmerge	0.073	0.435
Number of reflections	24177
<I/σ(I)>	13.6	3.6
Completeness [%]	96.3	99.4
Redundancy	5.5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	292	1.2M Na/K tartrate, 100 mM Tris pH8, VAPOR DIFFUSION, temperature 292K