4FPP
Bacterial phosphotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98011 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 103.540, 210.160, 93.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.970 - 2.200 |
| R-factor | 0.22971 |
| R-free | 0.26376 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.034 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.099 | 0.552 |
| Number of reflections | 52206 | |
| <I/σ(I)> | 12.5 | 2.88 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.7 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 24% PEG 4000, 0.16M magnesium chloride, 0.08M Tris-HCl pH8.5, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
