4FP7

2.2A resolution structure of Proteasome Assembly Chaperone Hsm3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-11-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	85.148, 94.466, 129.931
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.391 - 2.200
R-factor	0.1906
Rwork	0.188
R-free	0.23900
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.125
Data reduction software	XDS
Data scaling software	SCALA (CCP4_3.3.20)
Phasing software	PHASER (2.4.0)
Refinement software	PHENIX (dev_906)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	129.930	129.930	2.320
High resolution limit [Å]	2.200	6.960	2.200
Rmerge	0.097	0.030	0.660
Total number of observations		11224	52673
Number of reflections	53064
<I/σ(I)>	15.0771	41.76	2.97
Completeness [%]	98.6	98.27	97.78
Redundancy	6.75	6.08	6.93

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	20% PEG 4000, 100 mM Hepes, 150 mM ammonium sulfate, pH 7.0, vapor diffusion, temperature 293K