4FP5
Heat-labile enterotoxin ILT-IIbB5 S74A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2011-04-21 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.410, 67.585, 155.554 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.140 - 1.400 |
| R-factor | 0.1864 |
| Rwork | 0.185 |
| R-free | 0.21086 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q5b |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.447 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.410 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.065 | 0.330 |
| Number of reflections | 96076 | |
| <I/σ(I)> | 14.1 | 4.2 |
| Completeness [%] | 98.8 | 100 |
| Redundancy | 6.1 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 287 | 100 mM Bis-Tris propane, pH8.5, 20% PEG3350 and 200 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
