4FOM
Crystal structure of human nectin-3 full ectodomain (D1-D3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 131.867, 131.867, 247.543 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.930 |
| R-factor | 0.24824 |
| Rwork | 0.247 |
| R-free | 0.27904 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3alp |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.295 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 5.330 | 4.070 |
| High resolution limit [Å] | 3.930 | 4.950 | 3.930 |
| Number of reflections | 11776 | ||
| <I/σ(I)> | 34 | 3.2 | |
| Completeness [%] | 98.3 | 99 | 99.1 |
| Redundancy | 5.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293.15 | 4.6 M ammonium acetate, 0.1 M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
