4FOK
1.8 A Crystal structure of the FimX EAL domain in complex with c-diGMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-30 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.437 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 65.676, 85.515, 46.323 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.340 - 1.800 |
| R-factor | 0.157 |
| Rwork | 0.155 |
| R-free | 0.19786 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.548 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 24851 | |
| <I/σ(I)> | 16.56 | 3.25 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 7.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 291 | 0.6 M amonium sulfate, 0.1 M sodium citrate, pH 4.7, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
