4FOI
Crystal Structure of recombinant human Hexokinase type I mutant D413N with Glucose 1,6-bisphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.380, 120.772, 120.591 |
| Unit cell angles | 90.00, 92.80, 90.00 |
Refinement procedure
| Resolution | 35.410 - 2.400 |
| R-factor | 0.2484 |
| Rwork | 0.247 |
| R-free | 0.27290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.070 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.410 | 2.463 |
| High resolution limit [Å] | 2.400 | 2.401 |
| Number of reflections | 86070 | |
| Completeness [%] | 98.5 | 95.11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 6 | 277 | PEG 4000, PEG 8000, sodium acetate, sodium citrate, pH 6.0, hanging drop, temperature 277K |
