4FNP
Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 150.500, 150.500, 234.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.833 - 2.803 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.24710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.656 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.437 | |
| Number of reflections | 75205 | |
| <I/σ(I)> | 11.28 | 3.85 |
| Completeness [%] | 98.9 | 96.2 |
| Redundancy | 4.1 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 292 | 20% PEG 3350, 0.2M sodium iodide, 10% ethylene glycol, 5% polyvinyl pyrrolidone K15, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
