4FNO
Crystal structure of peptidyl t-RNA hydrolase from Pseudomonas aeruginosa at 2.2 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2012-05-22 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 61 |
Unit cell lengths | 62.604, 62.604, 155.392 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 54.220 - 2.250 |
R-factor | 0.20717 |
Rwork | 0.205 |
R-free | 0.25087 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4erx |
RMSD bond length | 0.021 |
RMSD bond angle | 1.836 |
Data reduction software | AUTOMAR |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.220 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 16116 | |
<I/σ(I)> | 5.9 | 5.9 |
Completeness [%] | 98.7 | 88.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M HEPES, PEG 4K, 5% Isopropanol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |