4FNK
Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-17 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.066 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 105.149, 151.458, 347.751 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.064 - 1.901 |
| R-factor | 0.1629 |
| Rwork | 0.162 |
| R-free | 0.18850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2viu |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.422 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 215875 | |
| <I/σ(I)> | 14.1 | 1.8 |
| Completeness [%] | 99.6 | 97.3 |
| Redundancy | 6.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 2.0 M ammonium sulfate, 100 mM sodium cacodylate, pH 6.5, 200 mM sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
