4FNJ
Utilizing the GAAA tetraloop/receptor to facilitate crystal packing and structure determination of a CUG RNA helix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97740 |
| Spacegroup name | H 3 |
| Unit cell lengths | 70.336, 70.336, 68.236 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.750 - 1.950 |
| R-factor | 0.21079 |
| Rwork | 0.208 |
| R-free | 0.26642 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 2.256 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.654 | |
| Number of reflections | 9054 | |
| <I/σ(I)> | 2.159 | |
| Completeness [%] | 99.3 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | 0.004M MgSO4, 0.05M Tris, 30% 1,6-hexanediol, VAPOR DIFFUSION, HANGING DROP, temperature 296K, pH 8.5 |
