4FNH
Crystal structure of IsdI-W66Y in complex with heme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-16 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.490, 68.800, 73.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.330 - 1.900 |
| R-factor | 0.19505 |
| Rwork | 0.193 |
| R-free | 0.22972 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lgn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.324 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.120 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.135 |
| Number of reflections | 24334 |
| <I/σ(I)> | 10.4 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | Crystals were grown in a reservoir solution containing 25% w/v polyethylene glycol 3350, 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 5.0, and flash frozen with a cryoprotectant of 20% ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
