4FNF
LT-IIB-B5 S74D mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2009-04-21 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.975 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.924, 70.648, 121.630 |
| Unit cell angles | 90.00, 117.29, 90.00 |
Refinement procedure
| Resolution | 30.820 - 1.750 |
| R-factor | 0.237 |
| Rwork | 0.234 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qb5 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.375 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.600 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.100 | 0.100 |
| Number of reflections | 99371 | |
| <I/σ(I)> | 6.2 | 6.2 |
| Completeness [%] | 98.6 | 98.6 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 8 | 287 | 20% PEG400, 100 mM K2HPO4, 100 mM Na acetate, pH 5.0, microbatch under oil, temperature 287K |
