4FN5
ELONGATION FACTOR G 1 (PSEUDOMONAS AERUGINOSA) IN COMPLEX WITH Argyrin B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-08-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.780, 88.100, 74.320 |
| Unit cell angles | 90.00, 107.91, 90.00 |
Refinement procedure
| Resolution | 55.390 - 2.900 |
| R-factor | 0.2068 |
| Rwork | 0.202 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.061 | 0.500 |
| Number of reflections | 16883 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 3.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | PROTEIN SOLUTION WAS INCUBATED WITH LIGAND FOR 1 HOUR. EQUAL VOLUMES OF RESERVOIR SOLUTION AND PROTEIN SOLUTION WERE MIXED. Reservoir Solution: 100MM TRIS HCL PH 7.5, 18% PEG3350, 0.2M Sodium NITRATE. PROTEIN SOLUTION: 50MM TRIS HCL PH7.5, 150MM NACL, 1MM TCEP , VAPOR DIFFUSION, SITTING DROP, temperature 294K |
