4FN0
Crystal structure of mouse nectin-2 extracellular fragment D1-D2, 2nd crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-30 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 117.022, 117.022, 158.467 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.350 |
| R-factor | 0.24921 |
| Rwork | 0.247 |
| R-free | 0.29333 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fmk |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.643 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 4.550 | 3.470 |
| High resolution limit [Å] | 3.350 | 4.220 | 3.350 |
| Number of reflections | 18438 | ||
| <I/σ(I)> | 18.7 | 4 | |
| Completeness [%] | 99.2 | 99.9 | 99.4 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293.15 | 13% w/v PEG3350, 0.42 M sodium isothiocyanate, 0.1 M MES, pH 6.0, cryoprotectant: 15% 2R,3R-butane-di-ol, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
