4FMV

Crystal Structure Analysis of a GH30 Endoxylanase from Clostridium papyrosolvens C71

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	105
Detector technology	IMAGE PLATE
Collection date	2011-05-15
Detector	RIGAKU RAXIS
Wavelength(s)	1.542
Spacegroup name	C 2 2 21
Unit cell lengths	66.020, 76.520, 150.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.260 - 2.010
R-factor	0.166
Rwork	0.164
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.416
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	75.275	38.260	2.120
High resolution limit [Å]	2.010	6.350	2.010
Rmerge		0.026	0.129
Number of reflections	24541
<I/σ(I)>	27.6	14.3	5.4
Completeness [%]	95.0	99.6	84.4
Redundancy	6.1	6.6	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M Ammonium acetate, 0.1M BIS-TRIS, 17% PEG10,000, pH pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K