4FMS
Crystal structure of Pseudomonas aeruginosa OccK2 (OpdF) in complex with glucuronate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.435, 206.802, 51.747 |
| Unit cell angles | 90.00, 99.09, 90.00 |
Refinement procedure
| Resolution | 14.992 - 2.450 |
| R-factor | 0.1936 |
| Rwork | 0.191 |
| R-free | 0.24250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.174 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.516 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 34356 | |
| Completeness [%] | 89.5 | 90 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 25% PEG 1K, 50 mM glucuronate, 50 mM Li2SO4, 50 mM Na2SO4 and 50 mM Na-acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 100K |
